| Virginia
Journal of Science.
2001. Vol 52 (1) :57-65 |
| Isolation
and Fusion of Cotton Protoplasts Michael H. Renfroe1, Ronald C. Hartwig2, and Roberta H. Smith2, 1Department of Biology, James Madison University, Harrisonburg, VA 22807, 2Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843 ABSTRACT
Protoplasts were isolated from five species of Gossypium. Protoplast yield
and viability were affected by incubation conditions, osmolarity,
purification procedures, and cell source. Using an optimized
procedure, highly viable protoplasts were isolated from cell
suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. klotzschianum, G. harknessii, and G. herbaceum. Protoplasts of G. harknessii were enucleated and
successfully fused with protoplasts of G. hirsutum. |